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PLoS Genet. 2014 Oct 9;10(10):e1004632. doi: 10.1371/journal.pgen.1004632. eCollection 2014 Oct.

Genome-wide mapping of yeast RNA polymerase II termination.

Author information

1
Department of Molecular Biology and Genetics, Johns Hopkins Medical School, Baltimore, Maryland, United States of America.

Abstract

Yeast RNA polymerase II (Pol II) terminates transcription of coding transcripts through the polyadenylation (pA) pathway and non-coding transcripts through the non-polyadenylation (non-pA) pathway. We have used PAR-CLIP to map the position of Pol II genome-wide in living yeast cells after depletion of components of either the pA or non-pA termination complexes. We show here that Ysh1, responsible for cleavage at the pA site, is required for efficient removal of Pol II from the template. Depletion of Ysh1 from the nucleus does not, however, lead to readthrough transcription. In contrast, depletion of the termination factor Nrd1 leads to widespread runaway elongation of non-pA transcripts. Depletion of Sen1 also leads to readthrough at non-pA terminators, but in contrast to Nrd1, this readthrough is less processive, or more susceptible to pausing. The data presented here provide delineation of in vivo Pol II termination regions and highlight differences in the sequences that signal termination of different classes of non-pA transcripts.

PMID:
25299594
PMCID:
PMC4191890
DOI:
10.1371/journal.pgen.1004632
[Indexed for MEDLINE]
Free PMC Article

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