Format

Send to

Choose Destination
Indian J Pharmacol. 2014 Sep-Oct;46(5):531-7. doi: 10.4103/0253-7613.140587.

Chemopreventive potential of Apium leptophyllum (Pers.) against DMBA induced skin carcinogenesis model by modulatory influence on biochemical and antioxidant biomarkers in Swiss mice.

Author information

1
NIMS Institute of Pharmacy, NIMS University, Jaipur, Rajasthan, India.
2
Department of Pharmacology, NIMS Medical College, NIMS University, Jaipur, Rajasthan, India.
3
Department of Pharmacognosy and Phytochemistry, Vedica College of Pharmacy, Bhopal, Madhya Pradesh, India.

Abstract

AIM:

The study was designed to investigate the chemopreventive potential of flavonoidal fractions of Apium leptophyllum fruits (FFALF) on Swiss mice.

MATERIALS AND METHODS:

Skin tumor or papilloma was developed by topical application of DMBA (25 μg in 0.1 ml acetone) on intrascapular region of mice, twice weekly for 8 weeks. The animals were divided into six groups: Group I (vehicle control); group II (FFALF control, 5 mg/kg); group III (carcinogenic control, DMBA treated initially for 8 weeks); and group IV, V and VI as pre-treated group (FFALF 5, 10 and 20 mg/kg respectively for 16 weeks along with DMBA treatment). After the 16(th) week of treatment; the tumor morphology, skin histopathology, and biochemical and antioxidant biomarkers were measured and compared with carcinogenic control as well as vehicle control.

RESULTS:

The co-administration of FFALF with DMBA-treated groups showed significant (P ≤ 0.001) prevention against skin papilloma and normalized the status of lipid peroxidation with antioxidant biomarkers in a dose-dependent manner as compared to carcinogenic control.

CONCLUSIONS:

Thus, the present study suggests that the FFALF is non-carcinogenic and has chemopreventive potential on DMBA-induced carcinogenesis in mouse, which may be due to the modulation of cutaneous lipid peroxidation or enhancement of total antioxidant capacity.

KEYWORDS:

Apium leptophyllum; DMBA; antioxidant biomarker; chemoprevention; skin papilloma

PMID:
25298584
PMCID:
PMC4175891
DOI:
10.4103/0253-7613.140587
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
Loading ...
Support Center