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Stem Cells Transl Med. 2014 Nov;3(11):1269-74. doi: 10.5966/sctm.2014-0129. Epub 2014 Oct 8.

Concise review: animal substance-free human embryonic stem cells aiming at clinical applications.

Author information

1
Departments of Clinical Science, Intervention and Technology and Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden; Karolinska University Hospital, Stockholm, Sweden; Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, Singapore Outi.Hovatta@ki.se.
2
Departments of Clinical Science, Intervention and Technology and Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden; Karolinska University Hospital, Stockholm, Sweden; Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, Singapore.

Abstract

Human embryonic stem cells have been considered the gold standard as a cell source for regenerative medicine since they were first cultured in 1998. They are pluripotent and can form principally all the cells types in the body. They are obtained from supernumerary human in vitro fertilization embryos that cannot be used for infertility treatment. Following studies on factors regulating pluripotency and differentiation, we now have techniques to establish and effectively expand these cells in animal substance-free conditions, even from single cells biopsied from eight-cell stage embryos in chemically defined feeder-free cultures. The genetic stability and absence of tumorigenic mutations can be determined. There are satisfactory animal tests for functionality and safety. The first clinical trials are ongoing for two indications: age-related macular degeneration and spinal cord injury.

KEYWORDS:

Clinical translation; Embryonic stem cells; Pluripotent stem cells; Serum-free; Stem cell culture

PMID:
25298372
PMCID:
PMC4214851
DOI:
10.5966/sctm.2014-0129
[Indexed for MEDLINE]
Free PMC Article

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