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Nat Rev Genet. 2014 Dec;15(12):814-27. doi: 10.1038/nrg3798. Epub 2014 Oct 9.

High-resolution digital profiling of the epigenome.

Author information

1
Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N, Seattle, Washington 98109, USA.
2
1] Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N, Seattle, Washington 98109, USA. [2] Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N, Seattle, Washington 98109, USA.

Abstract

The widespread adoption of short-read DNA sequencing as a digital epigenomic readout platform has motivated the development of genome-wide tools that achieve base-pair resolution. New methods for footprinting and affinity purification of nucleosomes, RNA polymerases, chromatin remodellers and transcription factors have increased the resolution of epigenomic profiling by two orders of magnitude, leading to new insights into how the chromatin landscape affects gene regulation. These digital epigenomic tools have also been applied to directly profile both turnover kinetics and transcription in situ. In this Review, we describe how these new genome-wide tools allow interrogation of diverse aspects of the epigenome.

PMID:
25297728
DOI:
10.1038/nrg3798
[Indexed for MEDLINE]

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