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Physiol Rep. 2014 Oct 7;2(10). pii: e12142. doi: 10.14814/phy2.12142. Print 2014 Oct 1.

Effects of vitamin C and E supplementation on endogenous antioxidant systems and heat shock proteins in response to endurance training.

Author information

1
Department of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
2
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
3
Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", Rome, Italy.
4
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway Division of Cancer Medicine, Surgery and Transplantation, Oslo University Hospital, Oslo, Norway.
5
Department of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway Norwegian Olympic Sports Center, Oslo, Norway.

Abstract

Reactive oxygen and nitrogen species are important signal molecules for adaptations to training. Due to the antioxidant properties of vitamin C and E, supplementation has been shown to blunt adaptations to endurance training. In this study, we investigated the effects of vitamin C and E supplementation and endurance training on adaptations in endogenous antioxidants and heat shock proteins (HSP). Thirty seven males and females were randomly assigned to receive Vitamin C and E (C + E; C: 1000 mg, E: 235 mg daily) or placebo (PLA), and underwent endurance training for 11 weeks. After 5 weeks, a subgroup conducted a high intensity interval session to investigate acute stress responses. Muscle and blood samples were obtained to investigate changes in proteins and mRNA related to the antioxidant and HSP system. The acute response to the interval session revealed no effects of C + E supplementation on NFκB activation. However, higher stress responses to exercise in C + E group was indicated by larger translocation of HSPs and a more pronounced gene expression compared to PLA. Eleven weeks of endurance training decreased muscle GPx1, HSP27 and αB-crystallin, while mnSOD, HSP70 and GSH remained unchanged, with no influence of supplementation. Plasma GSH increased in both groups, while uric acid decreased in the C + E group only. Our results showed that C + E did not affect long-term training adaptations in the antioxidant- and HSP systems. However, the greater stress responses to exercise in the C + E group might indicate that long-term adaptations occurs through different mechanisms in the two groups.

KEYWORDS:

Antioxidant enzymes; NFκB; gene expression; stress proteins

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