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Nat Commun. 2014 Oct 7;5:5105. doi: 10.1038/ncomms6105.

Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity.

Author information

1
1] Unité de défense innée et inflammation, Institut Pasteur, 75015 Paris, France [2] INSERM U874, 75015 Paris, France [3] Université Pierre et Marie Curie, Cellule Pasteur UPMC, rue du Dr Roux, 75015 Paris, France.
2
1] Unité de défense innée et inflammation, Institut Pasteur, 75015 Paris, France [2] INSERM U874, 75015 Paris, France.
3
1] Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France [2] Service de Pneumologie, Hôpital Cochin, AP-HP, Paris, France.
4
Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275 Centre National de la Recherche Scientifique et Université de Nice Sophia Antipolis, 06560 Valbonne, France.
5
INSERM U 806, Université René Descartes, CRCM, Service de Pneumo-Pédiatrie, Hôpital Necker-Enfants Malades, 75015 Paris, France.
6
Institute of Mother and Child, CF Centre, 01211 Warsaw, Poland.
7
1] Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France [2] Service de Bactériologie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, 75014 Paris, France.

Abstract

Young cystic fibrosis (CF) patients' airways are mainly colonized by Staphylococcus aureus, while Pseudomonas aeruginosa predominates in adults. However, the mechanisms behind this infection switch are unclear. Here, we show that levels of type-IIA-secreted phospholipase A2 (sPLA2-IIA, a host enzyme with bactericidal activity) increase in expectorations of CF patients in an age-dependent manner. These levels are sufficient to kill S. aureus, with marginal effects on P. aeruginosa strains. P. aeruginosa laboratory strains and isolates from CF patients induce sPLA2-IIA expression in bronchial epithelial cells from CF patients (these cells are a major source of the enzyme). In an animal model of lung infection, P. aeruginosa induces sPLA2-IIA production that favours S. aureus killing. We suggest that sPLA2-IIA induction by P. aeruginosa contributes to S. aureus eradication in CF airways. Our results indicate that a bacterium can eradicate another bacterium by manipulating the host immunity.

PMID:
25290234
DOI:
10.1038/ncomms6105
[Indexed for MEDLINE]

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