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Plast Reconstr Surg Glob Open. 2014 Jul 9;2(6):e162. doi: 10.1097/GOX.0000000000000109. eCollection 2014 Jun.

A New Economic Method for Preparing Platelet-rich Plasma.

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1
Tsurumai Kouen Clinic, Nagoya, Japan.

Abstract

BACKGROUND:

Although platelet-rich plasma (PRP) is nowadays a common method in various medical fields, including cosmetic surgery or dermatology, the expensiveness of the kit for processing is still a hurdle.

METHODS:

A new unique economic method for preparing PRP was reported. The method consists in a simple modification of a disposable 5-mL syringe that allows insertion into a common centrifuge and positioning of the syringe on the centrifuge so the PRP separates next to the tip of the syringe. Platelet-derived growth factor BB in PRP was measured under anticoagulant dextrose solution A (ACD-A) or heparin as anticoagulant and with or without prostaglandin E1 (PGE1) as a platelet aggregation suppressant.

RESULTS:

The new method successfully created PRP with high platelet-derived growth factor BB in all conditions, and the highest value was obtained by using ACD-A and PGE1.

CONCLUSIONS:

The new method is useful, and the use of ACD-A and PGE1 is the most recommended.

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