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Int J Antimicrob Agents. 2014 Dec;44(6):528-32. doi: 10.1016/j.ijantimicag.2014.07.026. Epub 2014 Sep 18.

Virological and serological analysis of a recent Middle East respiratory syndrome coronavirus infection case on a triple combination antiviral regimen.

Author information

1
Department of Microbiology, University of Athens, Medical School, 75 Mikras Asias Street, 115 27 Athens, Greece.
2
Hellenic Centre for Disease Control and Prevention, Athens, Greece; Department of Internal Medicine, University of Athens Medical School, Athens, Greece.
3
Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
4
First Department of Respiratory Medicine, University of Athens Medical School and 'Sotiria' Chest Diseases Hospital, Athens, Greece.
5
Department of Microbiology, University of Athens, Medical School, 75 Mikras Asias Street, 115 27 Athens, Greece. Electronic address: atsakris@med.uoa.gr.

Abstract

Serological, molecular and phylogenetic analyses of a recently imported case of Middle East respiratory syndrome coronavirus (MERS-CoV) in Greece are reported. Although MERS-CoV remained detectable in the respiratory tract secretions of the patient until the fourth week of illness, viraemia was last detected 2 days after initiation of triple combination therapy with pegylated interferon, ribavirin and lopinavir/ritonavir, administered from Day 13 of illness. Phylogenetic analysis of the virus showed close similarity with other human MERS-CoVs from the recent Jeddah outbreak in Saudi Arabia. Immunoglobulin G (IgG) titres peaked 3 weeks after the onset of illness, whilst IgM levels remained constantly elevated during the follow-up period (second to fifth week of illness). Serological testing confirmed by virus neutralisation assay detected an additional case that was a close contact of the patient.

KEYWORDS:

Antiviral treatment; Coronavirus; MERS; MERS-CoV; Phylogeny; Serology

[Indexed for MEDLINE]

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