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Methods Mol Biol. 2015;1222:37-59. doi: 10.1007/978-1-4939-1594-1_4.

Nuclear transfer and transgenesis in the pig.

Author information

1
Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377, Munich, Germany.

Abstract

Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, SCNT using in vitro matured oocytes, and laparoscopic embryo transfer.

PMID:
25287337
DOI:
10.1007/978-1-4939-1594-1_4
[Indexed for MEDLINE]

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