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J Biosci Bioeng. 2015 Apr;119(4):427-9. doi: 10.1016/j.jbiosc.2014.09.001. Epub 2014 Oct 3.

Enhanced cellular content and lactate fraction of the poly(lactate-co-3-hydroxybutyrate) polyester produced in recombinant Escherichia coli by the deletion of σ factor RpoN.

Author information

1
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan; CREST, JST, Sanbancho, Chiyoda-ku, Tokyo, Japan.
2
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan.
3
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan; CREST, JST, Sanbancho, Chiyoda-ku, Tokyo, Japan. Electronic address: staguchi@eng.hokudai.ac.jp.

Abstract

A new approach at the transcriptional level was applied to lactate-based polyester production. Four σ factor disruptants, ΔrpoN, ΔrpoS, ΔfliA and ΔfecI, of Escherichia coli were used as hosts for poly(lactate-co-3-hydroxybutyrate) production from glucose. Among them, ΔrpoN caused dual positive effects of polymer production, enhanced cellular content and lactate fraction.

KEYWORDS:

Biobased plastic; Poly(lactic acid); Polyhydroxyalkanoate; Polyhydroxybutyrate; σ factor

PMID:
25283807
DOI:
10.1016/j.jbiosc.2014.09.001
[Indexed for MEDLINE]

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