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Exp Hematol. 2015 Jan;43(1):65-70. doi: 10.1016/j.exphem.2014.08.007. Epub 2014 Oct 2.

Alternative splicing of EKLF/KLF1 in murine primary erythroid tissues.

Author information

1
Department of Developmental & Regenerative Biology, Mount Sinai School of Medicine, New York, NY, United States.
2
Department of Pediatric Hematology-Oncology, Weill Cornell Medical College, New York, NY, United States.
3
Department of Developmental & Regenerative Biology, Mount Sinai School of Medicine, New York, NY, United States; Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY, United States; Tisch Cancer Institute, Mount Sinai School of Medicine, New York, NY, United States. Electronic address: james.bieker@mssm.edu.

Abstract

Alternative splicing has emerged as a vital way to expand the functional repertoire of a set number of mammalian genes. For example, such changes can dramatically alter the function and cellular localization of transcription factors. With this in mind, we addressed whether EKLF/KLF1 mRNA, coding for a transcription factor that plays a critical role in erythropoietic gene regulation, is alternatively spliced. We find that EKLF mRNA undergoes exon skipping only in primary tissues and that this splice variant (SV) remains at a very low level in both embryonic and adult erythroid cells, as well as during terminal differentiation. The resultant protein is truncated and partially encodes a non-erythroid Krüppel-like factor amino acid sequence. Its overexpression can alter full-length erythroid Krüppel-like factor function at selected promoters. We discuss these results in the context of stress and with respect to recent global studies on the role of alternative splicing during terminal erythroid differentiation.

PMID:
25283745
PMCID:
PMC4268327
DOI:
10.1016/j.exphem.2014.08.007
[Indexed for MEDLINE]
Free PMC Article

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