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J Dairy Sci. 2014 Dec;97(12):7334-43. doi: 10.3168/jds.2014-7912. Epub 2014 Oct 3.

Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315.

Author information

1
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, P. R. China.
2
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
3
Food and Nutritional Science, School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong, China. Electronic address: npshah@hku.hk.
4
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, P. R. China. Electronic address: weihua114@live.cn.

Abstract

The objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (EPS) from Bifidobacterium bifidum WBIN03 (B-EPS) and Lactobacillus plantarum R315 (L-EPS). The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. Inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. Both B-EPS and L-EPS had strong scavenging ability against DPPH and superoxide radicals at high concentration. The inhibitory effect of B-EPS on erythrocyte hemolysis was stronger than that of L-EPS in a concentration range from 0.30 to 1.00 mg/mL, whereas the hydroxyl scavenging ability of L-EPS (39.15 ± 0.58%) was significantly higher than that of 0.15 mg/mL ascorbic acid (24.33 ± 1.17%) and B-EPS (17.89 ± 3.30%) at 0.10 mg/mL. The inhibition of lipid peroxidation of 0.50 mg/mL B-EPS and L-EPS was 13.48 ± 1.74% and 12.43 ± 0.51%, respectively, values lower than that of ascorbic acid at the same concentration (23.20 ± 1.41%). Furthermore, all these abilities were enhanced in a concentration-dependent manner. Agar diffusion assay showed that both EPS exhibited antibacterial activities against tested pathogens such as Cronobacter sakazakii, Escherichia coli, Listeria monocytogenes, Staphyloccocus aureus, Candida albicans, Bacillus cereus, Salmonella typhimurium, and Shigella sonnei at 300 μg/mL. In conclusion, both EPS have antimicrobial and antioxidant activities and could have applications in the food industry.

KEYWORDS:

Bifidobacterium bifidum; Lactobacillus plantarum; antioxidative and antibacterial activity; exopolysaccharide

PMID:
25282420
DOI:
10.3168/jds.2014-7912
[Indexed for MEDLINE]
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