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J Microbiol Methods. 2014 Dec;107:80-3. doi: 10.1016/j.mimet.2014.09.007. Epub 2014 Oct 1.

Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis.

Author information

1
Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.
2
Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany. Electronic address: joerg.willenborg@tiho-hannover.de.
3
Department of Microbial Genetics, University of Tuebingen, Tuebingen, Germany.
4
Bacterial and Parasitic Diseases Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan; The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.

Abstract

The lack of knowledge about pathogenicity mechanisms of Streptococcus (S.) suis is, at least partially, attributed to limited methods for its genetic manipulation. Here, we established a Cre-lox based recombination system for markerless gene deletions in S. suis serotype 2 with high selective pressure and without undesired side effects.

KEYWORDS:

Cre–lox system; Markerless mutagenesis; Streptococcus suis

PMID:
25281472
DOI:
10.1016/j.mimet.2014.09.007
[Indexed for MEDLINE]

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