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Appl Environ Microbiol. 2014 Dec;80(24):7710-6. doi: 10.1128/AEM.02867-14. Epub 2014 Oct 3.

Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization.

Author information

1
Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
2
Institute of Food Research, Norwich, United Kingdom John Innes Centre, Norwich, United Kingdom.
3
Institute of Food Research, Norwich, United Kingdom.
4
Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium filip.vanimmerseel@ugent.be.

Abstract

Salmonella enterica serovar Enteritidis has developed the potential to contaminate table eggs internally, by colonization of the chicken reproductive tract and internalization in the forming egg. The serotype Enteritidis has developed mechanisms to colonize the chicken oviduct more successfully than other serotypes. Until now, the strategies exploited by Salmonella Enteritidis to do so have remained largely unknown. For that reason, a microarray-based transposon library screen was used to identify genes that are essential for the persistence of Salmonella Enteritidis inside primary chicken oviduct gland cells in vitro and inside the reproductive tract in vivo. A total of 81 genes with a potential role in persistence in both the oviduct cells and the oviduct tissue were identified. Major groups of importance include the Salmonella pathogenicity islands 1 and 2, genes involved in stress responses, cell wall, and lipopolysaccharide structure, and the region-of-difference genomic islands 9, 21, and 40.

PMID:
25281378
PMCID:
PMC4249237
DOI:
10.1128/AEM.02867-14
[Indexed for MEDLINE]
Free PMC Article

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