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Cell Stem Cell. 2014 Oct 2;15(4):410-415. doi: 10.1016/j.stem.2014.09.014.

The naive state of human pluripotent stem cells: a synthesis of stem cell and preimplantation embryo transcriptome analyses.

Author information

1
Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA, Los Angeles, CA 90095, USA. Electronic address: khuang1@gmail.com.
2
Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
3
Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA, Los Angeles, CA 90095, USA. Electronic address: gfan@mednet.ucla.edu.

Abstract

Here we use a systems biology approach to comprehensively assess the conservation of gene networks in naive pluripotent stem cells (PSCs) with preimplantation embryos. While gene networks in murine naive and primed pluripotent states are reproducible across data sets, different sources of human stem cells display high degrees of variation, partly reflecting disparities in culture conditions. Finally, naive gene networks between human and mouse PSCs are not well conserved and better resemble their respective blastocysts.

PMID:
25280217
PMCID:
PMC5507179
DOI:
10.1016/j.stem.2014.09.014
[Indexed for MEDLINE]
Free PMC Article

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