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Diagn Microbiol Infect Dis. 2014 Dec;80(4):285-6. doi: 10.1016/j.diagmicrobio.2014.09.004. Epub 2014 Sep 16.

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples.

Author information

1
Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA.
2
Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA; Vaccine Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA. Electronic address: kjerome@fhcrc.org.

Abstract

We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control.

KEYWORDS:

Digital PCR; Inhibition; Quantitative PCR; Stool

[Indexed for MEDLINE]

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