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J Biosci Bioeng. 2015 Mar;119(3):310-3. doi: 10.1016/j.jbiosc.2014.08.012. Epub 2014 Sep 29.

Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
2
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan. Electronic address: iohtsu@bs.naist.jp.
3
Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., 1-1 Suzuki-cho, Kawasaki, Kanagawa 210-8681, Japan.
4
MS Business Unit, Shimadzu Co., 1 Nishinokyo, Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan.
5
Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.

Abstract

We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the L-cysteine producer. E. coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in L-methionine biosynthesis.

KEYWORDS:

Escherichia coli; Metabolome analysis; Monobromobimane; l-Cysteine; yciW gene

PMID:
25277519
DOI:
10.1016/j.jbiosc.2014.08.012
[Indexed for MEDLINE]

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