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Nat Commun. 2014 Oct 3;5:5104. doi: 10.1038/ncomms6104.

Visualizing the replication of respiratory syncytial virus in cells and in living mice.

Author information

1
1] Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France [2] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France [3] AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie, Boulogne-Billancourt 92100, France.
2
Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
3
1] Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France [2] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France.
4
Infection Innovative Medicines Unit, AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, Massachusetts 02451, USA.
5
1] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France [2] AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie, Boulogne-Billancourt 92100, France.

Abstract

Respiratory syncytial virus (RSV) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. Expression of mCherry and Luc are correlated with infection rate, allowing the monitoring of RSV multiplication in cell culture. Replication of the Luc-encoding virus in living mice can be visualized by bioluminescent imaging, bioluminescence being detected in the snout and lungs of infected mice after nasal inoculation. We propose that these recombinant viruses are convenient and valuable tools for screening of compounds active against RSV, and can be used as an extremely sensitive readout for studying effects of antiviral therapeutics in living mice.

PMID:
25277263
DOI:
10.1038/ncomms6104
[Indexed for MEDLINE]

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