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Arch Toxicol. 2016 Jan;90(1):181-90. doi: 10.1007/s00204-014-1377-5. Epub 2014 Oct 2.

Di-(2-ethylhexyl) phthalate accelerates atherosclerosis in apolipoprotein E-deficient mice.

Author information

1
Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, 11221, Taiwan.
2
Department of Surgery, Ren-Ai Taipei City Hospital, Taipei, 10629, Taiwan.
3
Nuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu, 30013, Taiwan.
4
Cardiovascular Division, Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan.
5
Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, 11221, Taiwan. tslee@ym.edu.tw.

Abstract

Di-(2-ethylhexyl) phthalate (DEHP) is associated with atherosclerosis-related cardiovascular disease complications, but we lack direct evidence of its unfavorable effect on atherogenesis. In this study, we aimed to clarify in vivo and in vitro the contribution of DEHP to the development of atherosclerosis and its underlying mechanisms. Apolipoprotein E-deficient (apoE(-/-)) mice chronically treated with DEHP for 4 weeks showed exacerbated hyperlipidemia, systemic inflammation, and atherosclerosis. In addition, DEHP promoted low-density lipoprotein (LDL) oxidation, which led to inflammation in endothelial cells as evidenced by increased protein expression of pro-inflammatory mediators. Furthermore, chronic DEHP treatment increased hepatic cholesterol accumulation by downregulating the protein expression of key regulators in cholesterol clearance including LDL receptor, cholesterol 7α-hydrolase, ATP-binding cassette transporter G5 and G8, and liver X receptor α. Moreover, the adiposity and inflammation of white adipose tissues were promoted in DEHP-treated apoE(-/-) mice. In conclusion, DEHP may disturb cholesterol homeostasis and deregulate the inflammatory response, thus leading to accelerated atherosclerosis.

KEYWORDS:

Atherosclerosis; Cholesterol metabolism; DEHP; Inflammation; Obesity

PMID:
25270622
DOI:
10.1007/s00204-014-1377-5
[Indexed for MEDLINE]

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