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Sci Rep. 2014 Oct 1;4:6487. doi: 10.1038/srep06487.

A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals.

Author information

1
1] Key Laboratory for Advanced Materials and Institute of Fine Chemicals Institution, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China [2].
2
College of Public Health, Nantong University, Seyuan Road 9, Nantong, Jiangsu 226019, China.
3
Clinical laboratory, Nantong Tumor Hospital, West Qingnian Road 48, Nantong, Jiangsu 226006, China.
4
Cancer Institutes, Fudan University Shanghai Cancer Center, Dongan Road 270, Shanghai 200032, China.
5
Department of chemistry, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China.
6
Key Laboratory for Advanced Materials and Institute of Fine Chemicals Institution, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China.

Abstract

We report a novel peptide probe for the detection of neurokinin-1 receptor using disaggregation-caused signal enhancement. The probe was obtained via the aggregation of a modified substance P in a terpyridine-Fe (II) complex with Gd (III)-DOTA into well-defined nanostructures, which effectively weaken ligand fluorescence and slow the exchange rate of inner-sphere water molecules. This probe disaggregates upon binding to the neurokinin-1 receptor and activates the contrast agents to generate a fluorescent signal that positively enhances magnetic resonance imaging contrast and allows for the detection of overexpressed receptors on tumor cells and the identification of lung cancer using serum samples.

PMID:
25270511
PMCID:
PMC4180826
DOI:
10.1038/srep06487
[Indexed for MEDLINE]
Free PMC Article

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