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Biochem Biophys Res Commun. 1989 Jul 31;162(2):583-90.

Deoxyribonucleoside triphosphate pools in mutagen sensitive mutants of Neurospora crassa.

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1
Department of Anatomy and Cell Biology, College of Veterinary Medicine, Texas A & M University, College Station 77843-4458.

Abstract

Deoxyribonucleoside triphosphate (dNTP) levels were measured in wild type Neurospora and nine mutagen-sensitive mutants, at nine different genes. Eight of these mutants are sensitive to hydroxyurea and histidine and show chromosomal instability, a phenotype which could result from altered levels of dNTPs. Two patterns were seen. Five of the mutants had altered ratios of dNTPs, with relatively high levels of dATP and dGTP and low levels of dCTP, but changes in the dTTP/dCTP ratio did not correlate with changes in spontaneous mutation levels. During exponential growth all but two of the mutants had small but consistent increases in dNTP pools compared to wild type. DNA content per microgram dry hyphae was altered in several mutants but these changes showed no correlation with the dNTP pool alterations.

PMID:
2527032
[Indexed for MEDLINE]
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