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Heredity (Edinb). 2015 May;114(5):431-40. doi: 10.1038/hdy.2014.86. Epub 2014 Oct 1.

Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation.

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Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
1] Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria [2] Center for Mind/Brain Sciences, University of Trento, Rovereto, Italy.
1] Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria [2] Vienna Graduate School of Population Genetics, Vienna, Austria.


Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.

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