Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation

Bernadette Crescenzo-Chaigne; Cyril Barbezange; Vianney Frigard; Damien Poulain; Sylvie van der Werf

doi:10.1371/journal.pone.0109046

Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation

PLoS One. 2014 Sep 30;9(9):e109046. doi: 10.1371/journal.pone.0109046. eCollection 2014.

Authors

Bernadette Crescenzo-Chaigne¹, Cyril Barbezange¹, Vianney Frigard¹, Damien Poulain¹, Sylvie van der Werf²

Affiliations

¹ Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France.
² Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France; Université Paris Diderot Sorbonne Paris Cité, Paris, France.

Abstract

Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5' NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Dogs
Gammainfluenzavirus / genetics*
Gammainfluenzavirus / metabolism
Gene Expression Regulation, Viral*
Influenza A virus / genetics*
Influenza A virus / metabolism
Madin Darby Canine Kidney Cells
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutant Chimeric Proteins / chemistry
Mutant Chimeric Proteins / genetics*
Mutant Chimeric Proteins / metabolism
Nucleocapsid Proteins
Protein Biosynthesis*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Viral / genetics
RNA, Viral / metabolism
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / genetics*
RNA-Binding Proteins / metabolism
Reverse Genetics
Transcription, Genetic
Viral Core Proteins / chemistry
Viral Core Proteins / genetics*
Viral Core Proteins / metabolism

Substances

Mutant Chimeric Proteins
NP protein, Influenza A virus
Nucleocapsid Proteins
RNA, Messenger
RNA, Viral
RNA-Binding Proteins
Viral Core Proteins

Grants and funding

This work was supported by institutional funds from the Institut Pasteur, the Centre National de la Recherche Scientifique and the University Paris Diderot, Sorbonne Paris Cité, Paris, France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.