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Plant Cell Physiol. 2015 Feb;56(2):242-54. doi: 10.1093/pcp/pcu134. Epub 2014 Sep 29.

Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan.
2
Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Saitama, 338-8570 Japan PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012 Japan.
3
RIKEN Plant Science Center, Yokohama, Kanagawa, 230-0045 Japan Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan.
4
Center for Frontier Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan.
5
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588 Japan.
6
College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880 Japan.
7
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045 Japan.
8
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045 Japan demura@bs.naist.jp.

Abstract

The secondary cell walls of xylem cells, including vessel elements, provide mechanical strength and contribute to the conduction of water and minerals. VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a NAC-domain transcription factor that regulates the expression of genes required for xylem vessel element formation. Transient expression assays using 68 transcription factors that are expressed during xylem vessel differentiation showed that 14 transcription factors, including VND1-VND7, are putative positive regulators of VND7 expression. Electrophoretic mobility shift assays revealed that all seven VND proteins bound to the VND7 promoter region at its SMBE/TERE motif, indicating that VND7 is a direct target of all of the VND transcription factors. Overexpression of VND1-VND5, GATA12 and ANAC075, newly identified transcription factors that function upstream of VND7, resulted in ectopic xylem vessel element formation. These data suggest that VND7 transcription is a regulatory target of multiple classes of transcription factors.

KEYWORDS:

Secondary cell wall; Transcription factor; Transcriptional network; Vascular development; Xylem vessel formation

PMID:
25265867
DOI:
10.1093/pcp/pcu134
[Indexed for MEDLINE]

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