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Nutr Cancer. 2014;66(8):1304-14. doi: 10.1080/01635581.2014.956246. Epub 2014 Sep 29.

Cytotoxic effects of ellagitannins isolated from walnuts in human cancer cells.

Author information

1
a Department of Biological Sciences , San José State University , San Jose , California , USA.

Abstract

Walnuts contain many bioactive components that may slow cancer growth. A previous report showed that a diet supplemented with walnuts decreased the tumor size formed by MDA-MB-231 human cancer cells injected into nude mice. However, the mechanism of action was never determined. We characterized the effects of a methanol extract prepared from walnuts on human MDA-MB-231, MCF7, and HeLa cells. The extract was cytotoxic to all cancer cells. We identified compounds from the methanol extract that induced this cytotoxicity. The predominant compounds were Tellimagrandin I and Tellimagrandin II, members of the ellagitannin family. We also show a walnut extract decreases the intracellular pH, depolarizes the mitochondrial membrane with release of cytochrome c and phosphatidylserine flipping. The antimitogenic effects of walnut extract were associated with a twofold reduction of mitochondria respiration. These results suggest impairment of mitochondrial function and apoptosis as relevant mechanism of anticancer effects of the walnut extract.

PMID:
25264855
DOI:
10.1080/01635581.2014.956246
[Indexed for MEDLINE]

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