Send to

Choose Destination
Cell Rep. 2014 Oct 9;9(1):32-39. doi: 10.1016/j.celrep.2014.08.052. Epub 2014 Sep 25.

Enteroendocrine cells support intestinal stem-cell-mediated homeostasis in Drosophila.

Author information

Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA; Department of Genetics,Harvard Medical School, Boston, MA 02115, USA.
Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, University of Strasbourg Institute for Advanced Study, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France.
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address:


Intestinal stem cells in the adult Drosophila midgut are regulated by growth factors produced from the surrounding niche cells including enterocytes and visceral muscle. The role of the other major cell type, the secretory enteroendocrine cells, in regulating intestinal stem cells remains unclear. We show here that newly eclosed scute loss-of-function mutant flies are completely devoid of enteroendocrine cells. These enteroendocrine cell-less flies have normal ingestion and fecundity but shorter lifespan. Moreover, in these newly eclosed mutant flies, the diet-stimulated midgut growth that depends on the insulin-like peptide 3 expression in the surrounding muscle is defective. The depletion of Tachykinin-producing enteroendocrine cells or knockdown of Tachykinin leads to a similar although less severe phenotype. These results establish that enteroendocrine cells serve as an important link between diet and visceral muscle expression of an insulin-like growth factor to stimulate intestinal stem cell proliferation and tissue growth.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center