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Respir Physiol Neurobiol. 2015 Jan 1;205:7-15. doi: 10.1016/j.resp.2014.09.010. Epub 2014 Sep 26.

Evidence for respiratory neuromodulator interdependence after cholinergic disruption in the ventral respiratory column.

Author information

1
Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: cmuere@mcw.edu.
2
Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: sneumueller@mcw.edu.
3
Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: jumiller@mcw.edu.
4
Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: solesiak@mcw.edu.
5
Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: mhodges@mcw.edu.
6
Physical Therapy, Marquette University, Milwaukee, WI 53201, USA. Electronic address: lawrence.pan@marquette.edu.
7
Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Zablocki Veterans Affairs Medical Center, Milwaukee, WI 53295, USA. Electronic address: bforster@mcw.edu.

Abstract

Reverse dialysis of the muscarinic receptor antagonist, atropine (ATR, 50 mM), into the pre-Bötzinger Complex region of the ventral respiratory column (VRC) of awake and sleeping goats increases breathing frequency and serotonin (5-HT), substance P (SP), glycine, and GABA concentrations in the effluent dialysate. Herein, we report data from goats in which we reverse dialyzed 5 mM ATR or specific antagonists of M2 or M3 muscarinic receptors into the VRC. The effects on frequency of all three antagonists were not significantly different from time control studies. 5 mM ATR and the M3 antagonist increased SP sevenfold less than 50 mM ATR. The antagonists had no effect on 5-HT, glycine, and/or GABA, suggesting that the increases in glycine and GABA with 50 mM ATR were secondary to the larger increases in 5-HT and/or SP. These data are suggestive of neuromodulator interdependence, whereby attenuation of one neuromodulator is compensated for by local changes in other neuromodulators to stabilize breathing.

KEYWORDS:

Control of breathing; Muscarinic receptor; Neuromodulator interdependence

PMID:
25262584
PMCID:
PMC4341919
DOI:
10.1016/j.resp.2014.09.010
[Indexed for MEDLINE]
Free PMC Article

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