Format

Send to

Choose Destination
Nat Methods. 2014 Nov;11(11):1161-9. doi: 10.1038/nmeth.3112. Epub 2014 Sep 28.

Intracellular autofluorescence: a biomarker for epithelial cancer stem cells.

Author information

1
Stem Cells and Cancer Group, Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
2
Melanoma Group, Molecular Pathology Programme, CNIO, Madrid, Spain.
3
Confocal Microscopy Unit, Biotechnology Programme, CNIO, Madrid, Spain.
4
Gastrointestinal Cancer Clinical Research Unit, Clinical Research Programme, CNIO, Madrid, Spain.
5
Chirurgische Klinik, Technische Universität München, Munich, Germany.
6
Università degli Studi di Verona, Centro Ricerca Applicata ARC-NET, Verona, Italy.
7
1] Stem Cells and Cancer Group, Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [2] Centre for Stem Cells in Cancer &Ageing, Barts Cancer Institute, Queen Mary University of London, London, UK.

Abstract

Cancer stem cells (CSCs) are thought to drive tumor growth, metastasis and chemoresistance. Although surface markers such as CD133 and CD44 have been successfully used to isolate CSCs, their expression is not exclusively linked to the CSC phenotype and is prone to environmental alteration. We identified cells with an autofluorescent subcellular compartment that exclusively showed CSC features across different human tumor types. Primary tumor-derived autofluorescent cells did not overlap with side-population (SP) cells, were enriched in sphere culture and during chemotherapy, strongly expressed pluripotency-associated genes, were highly metastatic and showed long-term in vivo tumorigenicity, even at the single-cell level. Autofluorescence was due to riboflavin accumulation in membrane-bounded cytoplasmic structures bearing ATP-dependent ABCG2 transporters. In summary, we identified and characterized an intrinsic autofluorescent phenotype in CSCs of diverse epithelial cancers and used this marker to isolate and characterize these cells.

PMID:
25262208
DOI:
10.1038/nmeth.3112
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center