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Ann Clin Microbiol Antimicrob. 2014 Sep 5;13:32. doi: 10.1186/s12941-014-0032-6.

Antifungal activity of pomegranate peel extract and isolated compound punicalagin against dermatophytes.

Author information

1
Post graduation in Microbiology, Universidade Estadual de Londrina, Londrina, PR, Brazil. simone.r.foss@gmail.com.
2
Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil. cvnakamura@uem.br.
3
Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil. tunakamura@uem.br.
4
Departament of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil. dagcortez@uem.br.
5
Post graduation in Pharmaceutical Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil. eliharue@gmail.com.
6
Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil. bpdfilho@uem.br.

Abstract

BACKGROUND:

Dermatophyte species infect the epidermis and appendages, often with serious social and health-economic consequences. The hydroalcoholic extract of pomegranate fruit peel showed activity against the dermatophyte fungi Trichophyton mentagrophytes, T. rubrum, Microsporum canis and M. gypseum.

METHODS:

Hydroalcoholic extract was prepared with pomegranate peels. This crude extract was fractionated and submitted to liquid-liquid partition, resulting in an active fraction which was fractionated in a Sephadex LH-20 column, followed by a Lobar column. The structure of the active compound was established with the use of spectroscopic methods.

RESULTS:

The crude extract of pomegranate fruit peel showed activity against the dermatophytes Trichophyton mentagrophytes, T. rubrum, Microsporum canis, and M. gypseum, with MICs values of 125 μg/ml and 250 μg/ml, respectively for each genus. Punicalagin was isolated and identified by spectroscopic analysis. The crude extract and punicalagin showed activity against the conidial and hyphal stages of the fungi. The cytotoxicity assay showed selectivity for fungal cells than for mammalian cells.

CONCLUSIONS:

These results indicated that the crude extract and punicalagin had a greater antifungal activity against T. rubrum, indicating that the pomegranate is a good target for study to obtain a new antidermatophyte medicine.

PMID:
25260038
PMCID:
PMC4353666
DOI:
10.1186/s12941-014-0032-6
[Indexed for MEDLINE]
Free PMC Article

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