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Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1758-9. doi: 10.3109/19401736.2014.963799. Epub 2014 Sep 26.

Next generation sequencing yields the complete mitochondrial genome of the flathead mullet, Mugil cephalus cryptic species NWP2 (Teleostei: Mugilidae).

Author information

1
a Department of Environmental Biology and Fisheries Science , National Taiwan Ocean University , Keelung , Taiwan , ROC .
2
b Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan , ROC .
3
c Department of Chemistry , Chung Yuan Christian University , Chung-Li , Taiwan .
4
d WeThink Biotech INC , Taoyuan , Taiwan , ROC .
5
e Department of Medical Genetics , National Taiwan University Hospital , Taipei , Taiwan , ROC , and.
6
f Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan , ROC.

Abstract

In this study, the complete mitogenome sequence of Northwestern Pacific 2 (NWP2) cryptic species of flathead mullet, Mugil cephalus (Teleostei: Mugilidae) has been amplified by long-range PCR and sequenced by next-generation sequencing method. The assembled mitogenome, consisting of 16,686 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop was 909 bp length and was located between tRNA-Pro and tRNA-Phe. The overall base composition of NWP2 M. cephalus was 28.4% for A, 29.8% for C, 26.5% for T and 15.3% for G. The complete mitogenome may provide essential and important DNA molecular data for further phylogenetic and evolutionary analysis for flathead mullet species complex.

KEYWORDS:

Flathead mullet; mitogenome; next generation sequencing

PMID:
25259447
DOI:
10.3109/19401736.2014.963799
[Indexed for MEDLINE]

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