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Mol Cell Endocrinol. 2015 Jan 5;399:43-9. doi: 10.1016/j.mce.2014.09.017. Epub 2014 Sep 26.

A two miRNA classifier differentiates follicular thyroid carcinomas from follicular thyroid adenomas.

Author information

1
Institute of Automatic Control, Silesian University of Technology, ul.Akademicka 16, 44-100 Gliwice, Poland; Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland; Department of Clinical Science, University of Bergen, Postboks 7800, NO-5020 Bergen, Norway.
2
Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland; Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Pasteura 3, Warsaw, Poland.
3
Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland.
4
Department of General, Visceral and Vascular Surgery, University of Halle-Wittenberg, Halle (Saale), Germany.
5
Department of General, Visceral, and Transplantation Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
6
Department of Pathology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
7
Tumor Pathology Department, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland.
8
Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark.
9
IZKF Leipzig, University of Leipzig, Liebigstr.21, D-04103 Leipzig, Germany.
10
Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany. Electronic address: Ralf.Paschke@medizin.uni-leipzig.de.
11
Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany.

Abstract

The inherent diagnostic limitations of thyroid fine needle aspiration (FNA), especially in the "indeterminate" category, can be partially overcome by molecular analyses. We aimed at the identification of miRNAs that could be used to improve the discrimination of indeterminate FNAs. miRNA expression profiling was performed for 17 follicular carcinomas (FTCs) and 8 follicular adenomas (FAs). The microarray results underwent cross-comparison using three additional microarray data sets. Candidate miRNAs were validated by qPCR in an independent set of 32 FTCs and 46 FAs. Sixty-eight differentially expressed miRNAs were identified. Thirteen miRNAs could be confirmed by cross comparison. A two-miRNA-classifier was established improving the diagnostic applicability and resulted in a sensitivity of 82% and a specificity of 49%. We present a classifier that has the potential to be successfully evaluated in cytology material for its capability to discriminate (mutation negative) indeterminate cytologies and thereby improving the pre-surgical diagnostics of thyroid nodules.

KEYWORDS:

Classifier; Follicular thyroid adenoma; Follicular thyroid cancer

PMID:
25258301
DOI:
10.1016/j.mce.2014.09.017
[Indexed for MEDLINE]

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