Format

Send to

Choose Destination
Brain Res. 2014 Nov 17;1589:93-104. doi: 10.1016/j.brainres.2014.09.033. Epub 2014 Sep 23.

Neuroprotection by 6-(methylsulfinyl)hexyl isothiocyanate in a 6-hydroxydopamine mouse model of Parkinson׳s disease.

Author information

1
Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, via Irnerio 48, 40126 Bologna, Italy. Electronic address: fabiana.morroni@unibo.it.
2
Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, via Irnerio 48, 40126 Bologna, Italy.
3
Department for Life Quality Studies, Alma Mater Studiorum - University of Bologna, Corso d׳Augusto, 237, 47900 Rimini, Italy.

Abstract

A number of pathogenic factors have been implicated in the progression of Parkinson׳s disease (PD), including oxidative stress, mitochondrial dysfunction, inflammation, excitotoxicity, and signals mediating apoptosis cascade. 6-(methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a major component in wasabi, a very popular spice in Japan and a member of the Brassica family of vegetables. This study was designed to investigate the neuroprotective effects of 6-MSITC in a PD mouse model. Mice were treated with 6-MSITC (5mg/kg twice a week) for four weeks after the unilateral intrastriatal injection of 6-hydroxydopamine (6-OHDA). On the 28th day, 6-OHDA-injected mice showed behavioral impairments, a significant decrease in tyrosine hydroxylase (TH) and an increase in apoptosis. In addition, lesioned mice showed reduced glutathione levels and glutathione-S-transferase and glutathione reductase activities. Notably, 6-MSITC demonstrated neuroprotective effects in our experimental model strongly related to the preservation of functional nigral dopaminergic neurons, which contributed to the reduction of motor dysfunction induced by 6-OHDA. Furthermore, this study provides evidence that the beneficial effects of 6-MSITC could be attributed to the decrease of apoptotic cell death and to the activation of glutathione-dependent antioxidant systems. These findings may render 6-MSITC as a promising molecule for further pharmacological studies on the investigation for disease-modifying treatment in PD.

KEYWORDS:

6-(methylsulfinyl)hexyl isothiocyanate; 6-hydroxydopamine; Neuroprotection; Oxidative stress; Parkinson׳s disease

PMID:
25257035
DOI:
10.1016/j.brainres.2014.09.033
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center