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Fertil Steril. 2014 Nov;102(5):1244-53. doi: 10.1016/j.fertnstert.2014.07.1209. Epub 2014 Sep 22.

World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonisation Project: IV. Tissue collection, processing, and storage in endometriosis research.

Author information

1
Organ Systems, Department of Development and Regeneration, KU Leuven, Leuven, Belgium; Department of Obstetrics and Gynecology, Leuven University Fertility Center, University Hospital Leuven, Leuven, Belgium.
2
Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
3
Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; Boston Center for Endometriosis, Boston Children's Hospital and Brigham and Women's Hospital, Boston, Massachusetts.
4
Obstetrics and Gynecology Unit, San Raffaele Scientific Institute, Milano, Italy.
5
University of California San Francisco, San Francisco, California; World Endometriosis Research Foundation (WERF), London, United Kingdom.
6
World Endometriosis Research Foundation (WERF), London, United Kingdom.
7
World Endometriosis Research Foundation (WERF), London, United Kingdom; Palo Alto Medical Foundation Fertility Physicians of Northern California, Palo Alto, California.
8
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Oxford, United Kingdom; Endometriosis Care Centre Oxford, University of Oxford, Oxford, United Kingdom.
9
Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; Boston Center for Endometriosis, Boston Children's Hospital and Brigham and Women's Hospital, Boston, Massachusetts; Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts.
10
Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom; Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Oxford, United Kingdom; Endometriosis Care Centre Oxford, University of Oxford, Oxford, United Kingdom. Electronic address: krinaz@well.ox.ac.uk.

Abstract

OBJECTIVE:

To harmonize standard operating procedures (SOPs) and standardize the recording of associated data for collection, processing, and storage of human tissues relevant to endometriosis.

DESIGN:

An international collaboration involving 34 clinical/academic centers and three industry collaborators from 16 countries on five continents.

SETTING:

In 2013, two workshops were conducted followed by global consultation, bringing together 54 leaders in endometriosis research and sample processing from around the world.

PATIENT(S):

None.

INTERVENTION(S):

Consensus SOPs were based on: 1) systematic comparison of SOPs from 24 global centers collecting tissue samples from women with and without endometriosis on a medium or large scale (publication on >100 cases); 2) literature evidence where available, or consultation with laboratory experts otherwise; and 3) several global consultation rounds.

MAIN OUTCOME MEASURE(S):

Standard recommended and minimum required SOPs for tissue collection, processing, and storage in endometriosis research.

RESULT(S):

We developed "recommended standard" and "minimum required" SOPs for the collection, processing, and storage of ectopic and eutopic endometrium, peritoneum, and myometrium, and a biospecimen data collection form necessary for interpretation of sample-derived results.

CONCLUSION(S):

The EPHect SOPs allow endometriosis research centers to decrease variability in tissue-based results, facilitating between-center comparisons and collaborations. The procedures are also relevant to research into other gynecologic conditions involving endometrium, myometrium, and peritoneum. The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies. The SOPs will be reviewed based on investigator feedback and through systematic triannual follow-up. Updated versions will be made available at: http://endometriosisfoundation.org/ephect.

KEYWORDS:

EPHect; Endometriosis; standard operating procedures; standardization; tissue

PMID:
25256928
PMCID:
PMC4230778
DOI:
10.1016/j.fertnstert.2014.07.1209
[Indexed for MEDLINE]
Free PMC Article

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