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Carbohydr Polym. 2014 Nov 26;113:314-24. doi: 10.1016/j.carbpol.2014.07.025. Epub 2014 Jul 21.

Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata).

Author information

1
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.
2
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China. Electronic address: qauwcr@126.com.

Abstract

An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.

KEYWORDS:

Antioxidant activity; Cucurbita moschata; Polysaccharide; Response surface methodology; Ultrasound-assisted enzymatic extraction

PMID:
25256490
DOI:
10.1016/j.carbpol.2014.07.025
[Indexed for MEDLINE]

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