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PLoS One. 2014 Sep 25;9(9):e107454. doi: 10.1371/journal.pone.0107454. eCollection 2014.

Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation.

Author information

1
Respiratory Department, The Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, China; Division of Allergy and Clinical Immunology, Department of Internal Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
2
Division of Allergy and Clinical Immunology, Department of Internal Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
3
Division of Pediatric Rheumatology, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America.

Abstract

IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects of IL-22 in allergic asthma. To determine the role of IL-22 in ovalbumin-induced allergic inflammation we generated inducible lung-specific IL-22 transgenic mice. Transgenic IL-22 expression and signaling activity in the lung were determined. Ovalbumin (OVA)-induced pulmonary inflammation, immune responses, and airway hyperresponsiveness (AHR) were examined and compared between IL-22 transgenic mice and wild type controls. Following doxycycline (Dox) induction, IL-22 protein was readily detected in the large (CC10 promoter) and small (SPC promoter) airway epithelial cells. IL-22 signaling was evidenced by phosphorylated STAT3. After OVA sensitization and challenge, compared to wild type littermates, IL-22 transgenic mice showed decreased eosinophils in the bronchoalveolar lavage (BAL), and in lung tissue, decreased mucus metaplasia in the airways, and reduced AHR. Among the cytokines and chemokines examined, IL-13 levels were reduced in the BAL fluid as well as in lymphocytes from local draining lymph nodes of IL-22 transgenic mice. No effect was seen on the levels of serum total or OVA-specific IgE or IgG. These findings indicate that IL-22 has immune modulatory effects on pulmonary inflammatory responses in allergen-induced asthma.

PMID:
25254361
PMCID:
PMC4177833
DOI:
10.1371/journal.pone.0107454
[Indexed for MEDLINE]
Free PMC Article

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