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Fish Shellfish Immunol. 2014 Dec;41(2):389-401. doi: 10.1016/j.fsi.2014.09.020. Epub 2014 Sep 22.

Characterization, expression and antibacterial properties of apolipoproteins A from carp (Cyprinus carpio L.) seminal plasma.

Author information

1
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland. Electronic address: m.dietrich@pan.olsztyn.pl.
2
University of Veterinary Medicine in Hanover, Fish Disease Research Unit, Germany.
3
Jagiellonian University in Krakow, Institute of Zoology, Poland.
4
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland.

Abstract

Apolipoproteins A are multifunctional proteins that, in addition to contributing to lipid metabolism and transport, are associated with the innate immune system in fish. Using a three step isolation procedure consisting of affinity chromatography on Blue-Sepharose, delipidation and reverse phase HPLC we isolated apolipoproteins from carp seminal plasma and identified them as ApoA-I and Apo-14 kDa. Moreover, we provided the full-length cDNA sequence of ApoA-I encoding 257 amino acids including a 18 amino acid signal peptide and a 4 amino acid propeptide. Apolipoproteins corresponded to the most abundant proteins in carp seminal plasma. Both ApoA-I and Apo-14 kDa were represented by several proteoforms that differ both in molecular mass and isoelectric point. The proteoforms of ApoA-I characteristic for seminal plasma were distinguished from those of blood. Carp seminal plasma ApoA-I and Apo-14 kDa showed a high immunologic similarity to their counterparts in carp blood and seminal plasma of other Cyprinid species. The mRNA expression analysis and immunohistochemical study suggest synthesis and secretion of ApoA-I and Apo-14 kDa in the fish reproductive tract and suggest a role in spermatogenesis and the stabilization of sperm membrane. Moreover, ApoA-I displayed bactericidal activity against Escherichia coli and bacteriostatic activity against Aeromonas hydrophila which suggests that ApoA-I is associated with innate immune system of the fish reproductive tract.

KEYWORDS:

Antimicrobial properties; Apolipoprotein; Carp; Seminal plasma

PMID:
25251775
DOI:
10.1016/j.fsi.2014.09.020
[Indexed for MEDLINE]

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