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PLoS One. 2014 Sep 24;9(9):e108697. doi: 10.1371/journal.pone.0108697. eCollection 2014.

Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo.

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1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America.

Abstract

Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of GECIs promises important advantages, including homogeneous, repeatable, and stable expression without the need for invasive virus injections. Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice.

PMID:
25250714
PMCID:
PMC4177405
DOI:
10.1371/journal.pone.0108697
[Indexed for MEDLINE]
Free PMC Article
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