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Development. 2014 Oct;141(19):3619-26. doi: 10.1242/dev.113027.

Poised chromatin in the mammalian germ line.

Author information

1
Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA leschb@wi.mit.edu.
2
Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA Howard Hughes Medical Institute, Whitehead Institute, Cambridge, MA 02142, USA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

Poised (bivalent) chromatin is defined by the simultaneous presence of histone modifications associated with both gene activation and repression. This epigenetic feature was first observed at promoters of lineage-specific regulatory genes in embryonic stem cells in culture. More recent work has shown that, in vivo, mammalian germ cells maintain poised chromatin at promoters of many genes that regulate somatic development, and that they retain this state from fetal stages through meiosis and gametogenesis. We hypothesize that the poised chromatin state is essential for germ cell identity and function. We propose three roles for poised chromatin in the mammalian germ line: prevention of DNA methylation, maintenance of germ cell identity and preparation for totipotency. We discuss these roles in the context of recently proposed models for germline potency and epigenetic inheritance.

KEYWORDS:

Bivalent; Chromatin; Germ cell; Germ line; Pluripotent; Poised

PMID:
25249456
PMCID:
PMC4197577
DOI:
10.1242/dev.113027
[Indexed for MEDLINE]
Free PMC Article

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