Format

Send to

Choose Destination
Plant Physiol. 2014 Nov;166(3):1280-7. doi: 10.1104/pp.114.244046. Epub 2014 Sep 23.

Rapid identification of a natural knockout allele of ARMADILLO REPEAT-CONTAINING KINESIN1 that causes root hair branching by mapping-by-sequencing.

Author information

1
Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.).
2
Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.) martin.huelskamp@uni-koeln.de andrea.schrader@uni-koeln.de.

Abstract

In Arabidopsis (Arabidopsis thaliana), branched root hairs are an indicator of defects in root hair tip growth. Among 62 accessions, one accession (Heiligkreuztal2 [HKT2.4]) displayed branched root hairs, suggesting that this accession carries a mutation in a gene of importance for tip growth. We determined 200- to 300-kb mapping intervals using a mapping-by-sequencing approach of F2 pools from crossings of HKT2.4 with three different accessions. The intersection of these mapping intervals was 80 kb in size featuring not more than 36 HKT2.4-specific single nucleotide polymorphisms, only two of which changed the coding potential of genes. Among them, we identified the causative single nucleotide polymorphism changing a splicing site in ARMADILLO REPEAT-CONTAINING KINESIN1. The applied strategies have the potential to complement statistical methods in high-throughput phenotyping studies using different natural accessions to identify causative genes for distinct phenotypes represented by only one or a few accessions.

PMID:
25248719
PMCID:
PMC4226369
DOI:
10.1104/pp.114.244046
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center