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Iran J Reprod Med. 2012 Nov;10(6):555-60.

Effects of prenatal exposure to extremely low electro-magnetic field on in vivo derived blastocysts of mice.

Author information

  • 1Department of Anatomy, Arak University of Medical Sciences, Arak, Iran.
  • 2Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
  • 3Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

BACKGROUND:

Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields.

OBJECTIVE:

The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the number and quality of mice blastocysts.

MATERIALS AND METHODS:

In present study, 66 NMRI pregnant females divided into two treated and non-treated groups. The treated group exposed to ELF-EMF (50 Hz and 6×10(-3) T). Subsequently, embryos were collected by flushing the uterine horn and Fallopian tubes on the day 3 of gestation. Number of trophoectoderm (TE) and Inner Cell Mass (ICM) cells in blastocysts were determined after differential nuclei staining using a modified method. Furthermore, number of all flushed blastocysts calculated in each group.

RESULTS:

There was no significant difference in mean number of blastocysts in treated (6.64±1.34) and none treated (8.22±1.59) groups. In treated group, there were significant decreased in total cell number of blastocysts (p=0.000), number of ICM cells (p=0.000), and number of TE cells (p=0.001) whereas the ratio of ICM/TE cells increased (p=0.002).

CONCLUSION:

The data indicate that ELF-EMF is able to affect cellular composition of blastocysts, but it can't omit total volume of blastocysts.

KEYWORDS:

Blastocyst; Electromagnetic fields; Mice

PMID:
25246926
PMCID:
PMC4169849
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