Format

Send to

Choose Destination
Nat Rev Genet. 2014 Nov;15(11):749-63. doi: 10.1038/nrg3803. Epub 2014 Sep 23.

Sequencing pools of individuals - mining genome-wide polymorphism data without big funding.

Author information

1
Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
2
1] Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria. [2] Vienna Graduate School of Population Genetics.

Abstract

The analysis of polymorphism data is becoming increasingly important as a complementary tool to classical genetic analyses. Nevertheless, despite plunging sequencing costs, genomic sequencing of individuals at the population scale is still restricted to a few model species. Whole-genome sequencing of pools of individuals (Pool-seq) provides a cost-effective alternative to sequencing individuals separately. With the availability of custom-tailored software tools, Pool-seq is being increasingly used for population genomic research on both model and non-model organisms. In this Review, we not only demonstrate the breadth of questions that are being addressed by Pool-seq but also discuss its limitations and provide guidelines for users.

PMID:
25246196
DOI:
10.1038/nrg3803
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center