Improved stability and cell response by intrinsic cross-linking of multilayers from collagen I and oxidized glycosaminoglycans

Biomacromolecules. 2014 Nov 10;15(11):4272-80. doi: 10.1021/bm501286f. Epub 2014 Oct 7.

Abstract

Stability of surface coatings against environmental stress, such as pH, high ionic strength, mechanical forces, and so forth, is crucial for biomedical application of implants. Here, a novel extracellular-matrix-like polyelectrolyte multilayer (PEM) system composed of collagen I (Col I) and oxidized glycosaminoglycans (oGAGs) was stabilized by intrinsic cross-linking due to formation of imine bonds between aldehydes of oxidized chondroitin sulfate (oCS) or hyaluronan (oHA) and amino groups of Col I. It was also found that Col I contributed significantly more to overall mass in CS-Col I than in HA-Col I multilayer systems and fibrillized particularly in the presence of native and oxidized CS. Adhesion and proliferation studies with murine C3H10T1/2 embryonic fibroblasts demonstrated that covalent cross-linking of oGAG with Col I had no adverse effects on cell behavior. By contrast, it was found that cell size and polarization was more pronounced on oGAG-based multilayer systems, which corresponded also to the higher stiffness of cross-linked multilayers as observed by studies with quartz crystal microbalance (QCM). Overall, PEMs prepared from oGAG and Col I give rise to stable PEM constructs due to intrinsic cross-linking that may be useful for making bioactive coatings of implants and tissue engineering scaffolds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cell Proliferation / drug effects
  • Cell Proliferation / physiology
  • Cells, Cultured
  • Collagen Type I / chemistry*
  • Collagen Type I / pharmacology
  • Cross-Linking Reagents / chemistry*
  • Cross-Linking Reagents / pharmacology
  • Glycosaminoglycans / chemistry*
  • Glycosaminoglycans / pharmacology
  • Mice
  • Mice, Inbred C3H
  • Oxidation-Reduction / drug effects
  • Tissue Scaffolds

Substances

  • Collagen Type I
  • Cross-Linking Reagents
  • Glycosaminoglycans