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Clin Chim Acta. 2015 Jan 1;438:304-6. doi: 10.1016/j.cca.2014.09.013. Epub 2014 Sep 19.

An abnormally glycosylated isoform of erythropoietin in hemangioblastoma is associated with polycythemia.

Author information

1
Department of Internal Medicine, AZ Maria Middelares, Ghent, Belgium.
2
Department of Laboratory Medicine, AZ Maria Middelares, Ghent, Belgium.
3
Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium.
4
VIB, Department of Medical Protein Research, Ghent University, Ghent, Belgium.
5
Department of Genetics, Ghent University Hospital, Ghent, Belgium.
6
Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium. Electronic address: joris.delanghe@ugent.be.

Abstract

BACKGROUND:

Hemangioblastomas express erythropoietin and the patients often present with polycythemia.

METHODS:

Serum erythropoietin was measured using a commercial immunoassay, a functional erythropoietin assay and iso-electric focusing.

RESULTS:

Despite the polycythemia, serum erythropoietin remained low, while a functional erythropoietin-assay showed a 4-5 higher activity in serum compared to the immunoassay. Iso-electric focusing of serum erythropoietin indicated overrepresentation of highly sialylated erythropoietin isoforms produced by the tumor. As a result, altered affinity of the monoclonal antibody used in the immunoassay for the hypersialylated isoforms was suggested.

CONCLUSION:

Analysis of erythropoietin isoforms may be helpful in distinguishing the ectopic erythropoietin isoforms from normally glycosylated erythropoietin.

KEYWORDS:

Erythropoietin isoforms; Glycosylation; Hemangioblastoma; von Hippel–Lindau

PMID:
25245675
DOI:
10.1016/j.cca.2014.09.013
[Indexed for MEDLINE]

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