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Braz J Microbiol. 2014 Aug 29;45(2):721-9. eCollection 2014.

Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism.

Author information

1
Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China.
2
Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Department of Biological and Environmental Engineering Hefei University Hefei China Department of Biological and Environmental Engineering, Hefei University, Hefei, China.
3
Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang China Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China.

Abstract

DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.

KEYWORDS:

DNJ Production; fermentation; microorganisms; mulberry

PMID:
25242964
PMCID:
PMC4166305
DOI:
10.1590/s1517-83822014000200048
[Indexed for MEDLINE]
Free PMC Article

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