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Braz J Microbiol. 2014 Aug 29;45(2):603-11. eCollection 2014.

Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66.

Author information

1
Plant Genomics Lab Department of Plant Sciences Quaid-i-Azam University Islamabad Pakistan Plant Genomics Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
2
National Institute for Genomics & Advanced Biotechnology National Agricultural Research Centre Islamabad Pakistan National Institute for Genomics & Advanced Biotechnology, National Agricultural Research Centre, Islamabad, Pakistan.
3
Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Abstract

Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p ≤ 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.

KEYWORDS:

Leclercia sp.; PGPR; bioinformatics; glucose dehydrogenase; pyrroloquinoline quinone

PMID:
25242947
PMCID:
PMC4166288
[Indexed for MEDLINE]
Free PMC Article

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