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Braz J Microbiol. 2014 Aug 29;45(2):603-11. eCollection 2014.

Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66.

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Plant Genomics Lab Department of Plant Sciences Quaid-i-Azam University Islamabad Pakistan Plant Genomics Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
National Institute for Genomics & Advanced Biotechnology National Agricultural Research Centre Islamabad Pakistan National Institute for Genomics & Advanced Biotechnology, National Agricultural Research Centre, Islamabad, Pakistan.
Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.


Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p ≤ 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.


Leclercia sp.; PGPR; bioinformatics; glucose dehydrogenase; pyrroloquinoline quinone

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