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Phys Chem Chem Phys. 2014 Nov 7;16(41):22841-52. doi: 10.1039/c4cp03102d.

The effect of myoglobin crowding on the dynamics of water: an infrared study.

Author information

1
Institut Rayonnement Matière de Saclay, LIDyL et Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux, UMR 3299, CNRS/CEA, Groupe Physico-Chimie sous Rayonnement, Bâtiment 546, F-91191 Gif-sur-Yvette Cedex, France. sophie.le-caer@cea.fr.

Abstract

Solutions containing 8 and 32 wt% myoglobin are studied by means of infrared spectroscopy, as a function of temperature (290 K and lower temperatures), in the mid- and far-infrared spectral range. Moreover, ultrafast time-resolved infrared measurements are performed at ambient temperature in the O-D stretching region. The results evidence that the vibrational properties of water remain the same in these myoglobin solutions (anharmonicity, vibrational relaxation lifetime…) and in neat water. However, the collective properties of the water molecules are significantly affected by the presence of the protein: the orientational time increases, the solid-liquid transition is affected in the most concentrated solution and the dynamical transition of the protein is observed, from the point of view of water, even in the least concentrated solution, proving that the water and myoglobin dynamics are coupled.

PMID:
25242637
DOI:
10.1039/c4cp03102d
[Indexed for MEDLINE]

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