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Methods Mol Biol. 2015;1206:123-36. doi: 10.1007/978-1-4939-1369-5_11.

RNA fluorescence in situ hybridization in cultured mammalian cells.

Author information

1
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Avenue, Urbana, IL, 61801, USA.

Abstract

It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

PMID:
25240892
DOI:
10.1007/978-1-4939-1369-5_11
[Indexed for MEDLINE]

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