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Nano Lett. 2014 Oct 8;14(10):5539-46. doi: 10.1021/nl501912g. Epub 2014 Sep 30.

Nanoparticle tension probes patterned at the nanoscale: impact of integrin clustering on force transmission.

Author information

1
Department of Chemistry, Emory University , 1515 Dickey Drive, Atlanta, Georgia 30322, United States.

Abstract

Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.

KEYWORDS:

Nanopatterning; focal adhesion; integrin; mechanotransduction; molecular tension fluorescence microscopy; receptor clustering

PMID:
25238229
PMCID:
PMC4189618
DOI:
10.1021/nl501912g
[Indexed for MEDLINE]
Free PMC Article

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