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Chem Biol. 2014 Sep 18;21(9):1171-84. doi: 10.1016/j.chembiol.2014.07.007.

Exploring metabolic pathways and regulation through functional chemoproteomic and metabolomic platforms.

Author information

1
Program in Metabolic Biology and Molecular Toxicology, Department of Nutritional Sciences and Toxicology, 127 Morgan Hall, Berkeley, CA 94720, USA.
2
Program in Metabolic Biology and Molecular Toxicology, Department of Nutritional Sciences and Toxicology, 127 Morgan Hall, Berkeley, CA 94720, USA. Electronic address: dnomura@berkeley.edu.

Abstract

Genome sequencing efforts have revealed a strikingly large number of uncharacterized genes, including poorly or uncharacterized metabolic enzymes, metabolites, and metabolic networks that operate in normal physiology, and those enzymes and pathways that may be rewired under pathological conditions. Although deciphering the functions of the uncharacterized metabolic genome is a challenging prospect, it also presents an opportunity for identifying novel metabolic nodes that may be important in disease therapy. In this review, we will discuss the chemoproteomic and metabolomic platforms used in identifying, characterizing, and targeting nodal metabolic pathways important in physiology and disease, describing an integrated workflow for functional mapping of metabolic enzymes.

PMID:
25237861
PMCID:
PMC4171689
DOI:
10.1016/j.chembiol.2014.07.007
[Indexed for MEDLINE]
Free PMC Article

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