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PLoS One. 2014 Sep 18;9(9):e107119. doi: 10.1371/journal.pone.0107119. eCollection 2014.

Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation.

Author information

1
Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, Georgia, United States of America.
2
Apeliotus Technologies, Inc., Atlanta, Georgia, United States of America.
3
Center for Biotechnology and Genomic Medicine, Department of Medicine, Medical College of Georgia at Georgia Regents University, Augusta, Georgia, United States of America.
4
Charlie Norwood VA Medical Center, Augusta, Georgia, United States of America; Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, Georgia, United States of America.

Abstract

We have previously shown that liposomes composed of egg-derived phosphatidylglycerol (PG), with a mixed fatty acid composition (comprising mainly palmitate and oleate), inhibit the proliferation and promote the differentiation of rapidly dividing keratinocytes, and stimulate the growth of slowly proliferating epidermal cells. To determine the species of PG most effective at modulating keratinocyte proliferation, primary mouse keratinocytes were treated with different PG species, and proliferation was measured. PG species containing polyunsaturated fatty acids were effective at inhibiting rapidly proliferating keratinocytes, whereas PG species with monounsaturated fatty acids were effective at promoting proliferation in slowly dividing cells. Thus, palmitoyl-arachidonyl-PG (16∶0/20∶4), palmitoyl-linoleoyl-PG (16∶0/18∶2), dilinoleoyl-PG (18∶2/18∶2) and soy PG (a PG mixture with a large percentage of polyunsaturated fatty acids) were particularly effective at inhibiting proliferation in rapidly dividing keratinocytes. Conversely, palmitoyl-oleoyl-PG (16∶0/18∶1) and dioleoyl-PG (18∶1/18∶1) were especially effective proproliferative PG species. This result represents the first demonstration of opposite effects of different species of a single class of phospholipid and suggests that these different PG species may signal to diverse effector enzymes to differentially affect keratinocyte proliferation and normalize keratinocyte proliferation. Thus, different PG species may be useful for treating skin diseases characterized by excessive or insufficient proliferation.

PMID:
25233484
PMCID:
PMC4169417
DOI:
10.1371/journal.pone.0107119
[Indexed for MEDLINE]
Free PMC Article

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